Recombinant Human BMP4  Protein 10ug expressed in human cells

Description

Summer Promotion
Recombinant
Human BMP4
Product Specifications
Expression of Human Proteins in Human Cells
Extreme low Endotoxin
High Purity
Animal Free and Xeno Free
Tag Free
Source:
Human cells derived
Structure:
Glycosylated homodimer
Purity:
>95% by SDS-PAGE
Endotoxin Level:
<0.5EU/ug
Molecular Weight:
34-40kDa
Formulation:
L
yophilized from a 0.2μm filtered solution in PBS without carrier protein
Activity Assay
The activity was measured by its ability to induce alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line).
Reconstitution
Briefly  centrifuge  the  vial  before  opening.  It  is recommended to reconstitute the protein in sterile
4 mM  HCl  containing  at  least  0.1%  human  or bovine serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
Protein Description
Bone morphogenetic protein 4 (BMP-4) is a member of transforming growth factor β family that includes more than 20 structurally related bone growth factors. BMP-4 is widely expressed from early embryogenesis through dulthood. It plays an important role in cartilage and bone formation, mesoderm induction, tooth development, limb formation and fracture repair. Mature human BMP-4 is a 116 amino acids glycoprotein and normally found as a homodimer. Dimerization is facilitated by a disulfide bridge formed between the monomer, which contains three intrachain disulfide bridges arranged in a cystine knot motif. BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.
References
Zhang Y, et al. (2008)Blood 111,1933.
Shore EM, et al. (1998)Calcif. Tissue Int. 63,221-229.
Nakamura K, et al. (1999)Exp. Cell Research 250,351-363.