Recombinant Human FLT3 Ligand 10ug expressed in human cells

Description

Summer Promotion
Recombinant Human FLT3L
Product Specifications
Expression of Human Proteins in Human Cells
Extreme low Endotoxin
High Purity
Animal Free and Xeno Free
T
ag Free
Source:
Human cells derived
Structure:
Glycosylated monomer
Purity:
>95% by SDS-PAGE
Endotoxin Level:
<0.5EU/ug
Molecular Weight:
24-30kDa
Formulation:
L
yophilized from a 0.2μm filtered solution in PBS without carrier protein
Activity Assay
The activity was measured by its ability to stimulate the proliferation of the human acute myeloid leukemia cell line OCI-AML5.
Reconstitution
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
Protein Description
Human Flt3 Ligand is a 24-30kDa glycoprotein with a 158 amino acid.Flt3 Ligand is expressed by T cells, bone marrow and thymic fibroblasts. The predominant biologically active form is membrane- bound isoform, which can be proteolytically cleaved to generate a biologically active soluble isoform. Flt3 Ligand synergizes well with a number of other colony stimulating factors and interleukins to regulate proliferation of early hematopoietic cells by activating Flt3.
References
Lyman SD. et al.1995 Oncogene. 10 (1), 149-57.
Hannum C., et al.(1994) Nature 368,643-648.
Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.